Laboratory stains, microorganism, microbiology, gram, wright, ziehlneelsen, lactophenol blue. Click here to visit our frequently asked questions about html5. The tb stain kit zn uses the ziehlneelsen hot acidfast procedure. Ziehlneelsen staining is a type of acidfast stain, first introduced by paul ehrlich. Used in the demonstration of acidfast bacteria belonging to the genus mycobacterium, which include the causative agent for tuberculosis. It is a special bacteriological stain used to identify acidfast organisms, mainly mycobacteria. When they are destained with acidalcohol, only nonacidfast bacteria get destained since they do not have a thick. Unlike the ziehlneelsen stain zn stain, the kinyoun method of staining does not require heating. The microscopic staining technique is one of the earliest methods devised for detecting the tubercle bacillus and it remains a standard. It is named for two german doctors who modified the stain. Your browser does not currently recognize any of the video formats available. Franz ziehl 1859 to 1926, a bacteriologist and friedrich neelsen 1854 to 1894, a pathologist. As a current student on this bumpy collegiate pathway, i stumbled. In this video we can see a tutorial for the determination of pulmonary tuberculosis, through the technique of bacilloscopy with ziehl neelsen staining.